Composite

Part:BBa_K3772010:Design

Designed by: Xiaoyu Wu   Group: iGEM21_SCUT-China   (2021-10-01)


PDC1p-VS-SAG1t expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 302


Design Notes

This sequence is codon optimized by Sangon Biotech. (https://www.sangon.com/services_gene_mmzyhhjysjfw.html) The parts are connected together by enzyme digestion


Source

PDC1p and SAG1t are cloned from the promoter of the coding sequence for aldehyde dehydrogenase from the genome of saccharomyces cereviciae BJ5464-NpgA； VS is synthesized by Sangon Biotech according to GenBank:JX040471.1；


References